Lambda Light Chain Antibody

• Description:

  This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

• Specifications:

  Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood, Immunofluorescence: 1-2 µg/mL

• UniProt:

  P01701, P01842

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Purified human IgG myeloma proteins were used as the immunogen for this Lambda Light Chain antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  HP6054

• Applications:

  FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  CF488 Conjugate

• Buffer:

  500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the Lambda Light Chain antibody at 2-8oC, protected from light.

• Limitations:

  This Lambda Light Chain antibody is available for research use only.

• Storage Conditions:

  Store the Lambda Light Chain antibody at 2-8°C, protected from light.

• Formulation:

  500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Lambda Light Chain antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic, secreted

• Image Legend:

  (Left) Immunofluorescent staining of permeabilized human Ramos cells with CF488-labeled Lambda Light Chain antibody (clone HP6054, green) and DAPI (blue) . (Right) Negative control.