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Lambda Light Chain Antibody

CAT: 0800-V3261-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V3261-100UGSize:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Individual B-cells in lymphoid tissue possess either kappa or lambda light chains, but never both together. Specific rearrangement of lambda light chain of immunoglobulins can lead to loss of some protein coding genes, which does not seem to be functionally relevant (while functionally relevant miR-650 can be overexpressed) . Using immunohistochemistry, it is possible to determine the relative abundance of B-cells expressing kappa and lambda light chains. If the lymph node or similar tissue is reactive, or otherwise benign, it should possess a mixture of kappa positive and lambda positive cells. If, however, one type of light chain is significantly more common than the other, the cells are likely all derived from a small clonal population, which may indicate a malignant condition, such as B-cell lymphoma. [Wiki]
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
P01701, P01842
Host
Mouse
Reactivity
Human
Immunogen
IgG purified from human serum was used as the immunogen for this Lambda Light Chain antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
N10/2
Applications
FACS, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Lambda Light Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Lambda Light Chain antibody is available for research use only.
Storage Conditions
Store the Lambda Light Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The optimal dilution of the Lambda Light Chain antibody for each application should be determined by the researcher.
Location
Cell Surface, Cytoplasmic and Secreted
Image Legend
IHC testing of FFPE human tonsil stained with Lambda Light Chain antibody (clone N10/2) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
Product Datasheet
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