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CDC2 Antibody / CDK1

CAT: 0800-V3040-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V3040-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. Cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated Cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL
UniProt
P06493
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the CDC2 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
CDK1/873
Applications
IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CDC2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CDC2 antibody is available for research use only.
Storage Conditions
Store the CDC2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CDC2 antibody should be determined by the researcher.
Location
Nuclear and cytoplasmic
Image Legend
IHC testing of formalin-fixed, paraffin-embedded human tonsil with CDC2 antibody (CDK1/873) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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