CDK1 Antibody / CDC2

• Description:

  This antibody recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (Cdk1) or p34cdc2 protein kinase. CDK1 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated CDK1 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

• Specifications:

  Western blot: 0.5-2 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT

• UniProt:

  P06493

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Purified recombinant human Cdk1 / p34cdc2 was used as the immunogen for this CDK1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  POH-1

• Applications:

  WB, IHC-P

• Purity:

  Protein G purified antibody

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the CDK1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CDK1 antibody is available for research use only.

• Storage Conditions:

  Store the CDK1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CDK1 antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

• CAS Number:

  9007-83-4

• Location:

  Nuclear and Cytoplasmic

• Image Legend:

  IHC testing of human tonsil stained with CDK1 antibody.