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CDK1 Antibody / CDC2

CAT: 0800-R31895Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31895Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CDC2, Cell Division Cycle 2, is also known as CDK1 (Cyclin-dependent Kinase 1) . CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10.
CAS Number
9007-83-4
Specifications
Western blot: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunofluorescence/Immunocytochemistry: 2-4 µg/mL, Flow cytometry: 1-3ug/10^6 cells
UniProt
P06493
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 66-297 of human CDK1/CDC2 were used as the immunogen for the CDK1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, IF/ICC
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the CDK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CDK1 antibody is available for research use only.
Storage Conditions
After reconstitution, the CDK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the CDK1 antibody should be determined by the researcher.
Location
Nuclear and cytoplasmic
Image Legend
Western blot testing of 1) rat thymus, 2) rat spleen, 3) human MCF7, 4) HeLa, 5) Jurkat, and 6) mouse NIH3T3 lysate with CDK1 antibody. Predicted/observed molecular weight: ~33 kDa.

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