CD36 Antibody

• Description:

  Recognizes a protein of 80kDa-90kDa, identified as CD36. It is expressed on platelets, monocytes and macrophages, microvascular endothelial cells, erythrocyte precursors, mammary epithelial cells, and some macrophage derived dendritic cells. CD36 acts as a receptor for thrombospondin (TSP), collagen types I, IV and V, P. falciparum malaria-infected erythrocytes, and sickle erythrocytes. It also functions as a scavenger receptor, mediating macrophage uptake of oxidized low-density lipoprotein (LDL) and recognition of apoptotic polymorphonuclear leukocytes (PMN) . CD36 plays a role in platelet aggregation, macrophage foam cell development, inflammation, and the tissue ischemia observed in sickle cell disease and cerebral malaria. Note that 1-4% of Japanese and East Asia population lack CD36.

• Specifications:

  Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL

• UniProt:

  P16671

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human protein was used as the immunogen for the CD36 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  GPIIIb/1654

• Applications:

  FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the CD36 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD36 antibody is available for research use only.

• Storage Conditions:

  Store the CD36 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the CD36 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  SDS-PAGE analysis of purified, BSA-free CD36 antibody (clone GPIIIb/1654) as confirmation of integrity and purity.