CD86 Antibody

• Description :

  CD86 is a receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. [UniProt]

• Specifications :

  Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT

• UniProt :

  P42081

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Recombinant human full-length protein was used as the immunogen for the CD86 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  SPM600

• Applications :

  IF, IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution :

  Store the CD86 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This CD86 antibody is available for research use only.

• Storage Conditions :

  Store the CD86 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the CD86 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic, membrane

• Image Legend :

  IHC: Formalin-fixed, paraffin-embedded human melanoma stained with CD86 antibody (clone SPM600) .