TNFa Antibody

• Description:

  This antibody is specific for a 17-26KD protein which is identified as the cytokine Tumor Necrosis Factor alpha. It is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Other actions of TNFa include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.

• Specifications:

  Western blot: 0.5-1 µg/mL

• UniProt:

  P16599

• Host:

  Rabbit

• Reactivity:

  Rat

• Immunogen:

  Rat partial recombinant protein (AA 89-235) was used as the immunogen for this TNFa antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the TNFa antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This TNFa antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the TNFa antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Titration of the TNFa antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of TNFa antibody and rat samples: 1. PC-12, 2. spleen, 3. brain, 4. kidney, 5. liver lysate. Predicted/observed molecular weight: ~26 kDa.