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TNF-a Antibody

CAT: 0013-GTR18572799Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18572799Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
UniProt
P01375
Reactivity
Human
Immunogen
Amino acids 115-130 (NGVELRDNQLVVPSEG) from the human protein were used as the immunogen for the TNF-a antibody.
Clonality
Monoclonal
Clone
P/T2
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
PEG precipitation
Dilution
Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the TNF-a antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
Tested Applications
FACS, IF, IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgM, kappa

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