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TGFa Antibody

CAT: 0800-V2885-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V2885-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this mAb is completely blocked by the peptide used for raising this antibody. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT
UniProt
P01135
Host
Mouse
Reactivity
Human
Immunogen
Amino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for the TGFa antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
TG86
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the TGFa antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This TGFa antibody is available for research use only.
Storage Conditions
Store the TGFa antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the TGFa antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Location
Cytoplasmic and extracellular (secreted)
Image Legend
IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with TGFa antibody (TG86)

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