Products for Research Use Only
RegisterLogin

Nucleolin Antibody Cocktail

CAT: 0800-V2758IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-V2758IHC-7MLSize:7 mL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL) . It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
CAS Number
9007-83-4
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
UniProt
P19338
Host
Mouse
Reactivity
Human
Immunogen
SUDHL1 cell nuclear lysate (364-5) and recombinant human protein (NCL/902) were used as the immunogen for the Nucleolin antibody cocktail.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
364-5 + NCL/902
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the Nucleolin antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Nucleolin antibody cocktail is available for research use only.
Storage Conditions
Store the Nucleolin antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Nucleoli
Image Legend
IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Nucleolin antibody (364-5 + NCL/902) .

UniProtKB · P19338

Nucleolin

NUCL_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P19338
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NCL
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q53SK1, Q8NB06, Q9UCF0, Q9UDG1
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondMethylationPhosphoproteinUbl conjugation

Cellular component

CytoplasmNucleus

Molecular function

DNA-bindingRNA-binding

Domain

Repeat

Related Products

CatalogName
V4963-100UGNucleolin Antibody
V7196IHC-7MLNucleolin Antibody
V2755SAF-100UGNucleolin Antibody
V4963-20UGNucleolin Antibody
V7196-20UGNucleolin Antibody
F40212-0.08MLNucleolin Antibody
V2755CF488-100TNucleolin Antibody
V2755-20UGNucleolin Antibody
V9444SAF-100UGNucleolin Antibody
V9444-20UGNucleolin Antibody

Alternative Products

CatalogName
GTR18572614Nucleolin Antibody Cocktail
MBS9435026-01Nucleolin Antibody
STJ140144Anti-Nucleolin antibody
052901Nucleolin

Popular Products