Nucleolin Antibody

CAT: 0800-V7196-20UGSize: 20 µgDry Ice: NoHazardous: No
CAT#:0800-V7196-20UGSize:20 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Nucleolin (NCL) is the major nucleolar phosphoprotein of growing eukaryotic cells. It is located mainly in dense fibrillar regions of the nucleolus. NCL is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells in 0.1ml, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
UniProt
P19338
Host
Mouse
Reactivity
Human, Mouse
Immunogen
Recombinant human protein was used as the immunogen for the Nucleolin antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
NPC23-2
Applications
FACS, IHC-P, WB, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Nucleolin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Nucleolin antibody is available for research use only.
Storage Conditions
Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nucleoli
Image Legend
IHC testing of FFPE human tonsil with Nucleolin antibody (clone NPC23-2) . FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

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