Nucleolin Antibody

• Description:

  Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. [UniProt]

• Specifications:

  Western blot: 1:500-1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:10-1:50

• UniProt:

  P19338

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  This Nucleolin antibody was produced from mice immunized with a protein from human NCL.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  130CT43.8.5

• Applications:

  WB, IHC, IF, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS pH 7.4 with 0.09% sodium azide

• Reconstitution:

  Aliquot the Nucleolin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Nucleolin antibody is available for research use only.

• Storage Conditions:

  Aliquot the Nucleolin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the Nucleolin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of Nucleolin antibody and 293 lysate. Predicted molecular weight is 77 kDa, observed size is 100~110 kDa.