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CD99 Antibody Cocktail

CAT: 0800-V2711S-0.1MLSize: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0800-V2711S-0.1MLSize:0.1 mL
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Description
Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32) . Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.
CAS Number
9007-83-4
Specifications
Flow cytometry: 5-10ul/10^6 cells, Immunofluorescence: 1:100-1:200, Immunohistochemistry (FFPE) : 1:100-1:200 for 30 min at RT
UniProt
P14209
Host
Mouse
Reactivity
Human
Immunogen
Human acute lymphocytic leukemia T-cells (12E7) and recombinant human protein (MIC2/877) were used as the immunogen for the CD99 antibody cocktail.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
12E7 + MIC2/877
Applications
FACS, IF, IHC-P
Purity
Unpurified high titer supernatant
Format
Bioreactor concentrate
Buffer
Bioreactor concentrate with 0.05% sodium azide
Reconstitution
Store the CD99 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD99 antibody cocktail is available for research use only.
Storage Conditions
Store the CD99 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Bioreactor concentrate with 0.05% sodium azide
Applications Notes
Optimal dilution of the CD99 antibody cocktail should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Location
Cell surface
Image Legend
IHC: Formalin-fixed, paraffin-embedded human Ewing's sarcoma stained with CD99 antibody (clones 12E7 + MIC2/877) . Required HIER: boil tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

UniProtKB · P14209

CD99 antigen

CD99_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P14209
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CD99
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A6NIW1, O00518, Q6ICV7
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Cell adhesion

PTM

GlycoproteinPhosphoprotein

Cellular component

Membrane

Domain

SignalTransmembraneTransmembrane helix

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