CD99 Antibody

• Description :

  CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, it may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of the protein. [Wiki]

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

• UniProt :

  P14209

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Recombinant full-length human protein was used as the immunogen for the CD99 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  CDLA99

• Applications :

  IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution :

  Store the CD99 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This CD99 antibody is available for research use only.

• Storage Conditions :

  Store the CD99 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number :

  9007-83-4

• Location :

  Cell surface

• Image Legend :

  IHC testing of FFPE human Ewing's sarcoma and CD99 antibody (clone CDLA99) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.