CD18 Antibody

• Description:

  It recognizes a transmembrane glycoprotein of 95kDa, identified as CD18 or integrin -2 (Workshop III) . It complexes non-covalently with either L, M, or X integrin (CD11a, b, or c) to form the heterodimers, LFA-1, Mac-1, and p150,95, respectively. LFA-1 is the receptor for three members of the Ig supergene family of proteins, ICAM-1 (CD54), ICAM-2 (CD102), and Mac-1 and p150,95 bind to ICAM-1, fibrinogen, and iC3b. ICAM-3 (CD50) . CD18/CD11 heterodimeric molecules are involved with cell/cell and cell/extracellular adhesion in immune and inflammatory responses. This mAb blocks these cellular interactions.

• Specifications:

  Functional studies (order BSA/sodium azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL

• UniProt:

  P05107

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Stimulated human leukocytes were used as the immunogen for the CD18 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  68-5A5

• Applications:

  Functional studies, FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the CD18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD18 antibody is available for research use only.

• Storage Conditions:

  Store the CD18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD18 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  SDS-PAGE Analysis of Purified, BSA-Free CD18 Antibody (clone 68-5A5) . Confirmation of Integrity and Purity of the Antibody.