CD19 Antibody

• Description :

  CD19 is expressed on follicular dendritic cells and B cells. It is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. [Wiki]

• Specifications :

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL

• UniProt :

  P15391

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Human CD19 recombinant full length protein was used as immunogen for this CD19 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  CVID3/429

• Applications :

  FACS, IF

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  CD19 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

• Limitations :

  This CD19 antibody is available for research use only.

• Storage Conditions :

  CD19 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  Variations in protocols, secondaries and substrates may require the CD19 antibody to be titered up or down for optimal performance.

• CAS Number :

  9007-83-4

• Location :

  Cell surface, cytoplasmic

• Image Legend :

  Immunofluorescent staining of human Raji cells with CD19 antibody (clone CVID3/429, green) and Reddot nuclear stain (red) .