CD15 Antibody

• Description:

  CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

• Specifications:

  Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

• UniProt:

  P22083

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Myelomonocytic leukemia cells were used as the immunogen for the CD15 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgM k

• Clone:

  BRA-4F1

• Applications:

  FACS, IF, IHC-P

• Purity:

  PEG precipitation

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the CD15 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD15 antibody is available for research use only.

• Storage Conditions:

  Store the CD15 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD15 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

• CAS Number:

  9007-83-4

• Location:

  Cell surface and granular paranuclear

• Image Legend:

  IHC: Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD15 antibody (BRA-4F1) .