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Anti-CD45RO Antibody

CAT: 0800-V2420IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
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CAT#:0800-V2420IHC-7MLSize:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) . This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
CAS Number
9007-83-4
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
UniProt
P08575
Host
Mouse
Reactivity
Human
Immunogen
Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as immunogen for this anti-CD45RO antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
SPM125
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
The anti-CD45RO antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Limitations
This anti-CD45RO antibody is available for research use only.
Storage Conditions
The anti-CD45RO antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Cell surface
Image Legend
IHC testing of anti-CD45RO antibody and FFPE human lymphoma

UniProtKB · P08575

Receptor-type tyrosine-protein phosphatase C

PTPRC_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P08575
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PTPRC
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.1.3.48
Processing
Precursor
Secondary accessions
A0A0A0MT22, A8K7W6, Q16614, Q9H0Y6, X6R433
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSynapse

Disease

Disease variantSCID

PTM

GlycoproteinPhosphoprotein

Biological process

Host-virus interaction

Molecular function

HydrolaseProtein phosphatase

Domain

RepeatSignalTransmembraneTransmembrane helix

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