CD45RO Antibody

• Description :

  Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) . This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

• Specifications :

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL

• UniProt :

  P08575

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Lentillectin purified glycoproteins obtained from HuPBL and from a CLL (B cell) were used as immunogen for this CD45RO antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2a κ

• Clone :

  190-2F2.5

• Applications :

  FACS, IF

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1X PBS, pH 7.4

• Reconstitution :

  The CD45RO antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

• Limitations :

  This CD45RO antibody is available for research use only.

• Storage Conditions :

  The CD45RO antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes :

  Due to variation in protocol and secondary antibody used, the CD45RO antibody may need to be titered for optimal performance.

• CAS Number :

  9007-83-4

• Location :

  Cell surface

• Image Legend :

  Immunofluorescent staining of PFA-fixed human Jurkat cells with CD45RO antibody (clone 190-2F2.5, green) and Reddot nuclear stain (red) .