CD45RO Antibody

• Description:

  CD45RO is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. CD45RO contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. It has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. It also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. [RefSeq]

• Specifications:

  Flow Cytometry: 0.5-1ug/million cells, Immunohistology (FFPE) : 0.25-0.5 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

• UniProt:

  P08575

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human protein was used as immunogen for this CD45RO antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  CDLA45RO-1

• Applications:

  FACS, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  The CD45RO antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

• Limitations:

  This CD45RO antibody is available for research use only.

• Storage Conditions:

  The CD45RO antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  Flow cytometry testing of PFA-fixed human Jurkat cells with CD45RO antibody (clone CDLA45RO-1) ; Red=isotype control, Blue= CD45RO antibody.