CD20 Antibody

• Description :

  Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

• Specifications :

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt :

  P11836

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Recombinant CD20 protein was used as the immunogen for this CD20 antibody. The epitope is localized to the cytoplasmic region of the protein.

• Clonality :

  Monoclonal

• Isotype :

  IgG2a κ

• Clone :

  IGEL/773

• Applications :

  FACS, IF, IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

• Limitations :

  This CD20 antibody is available for research use only.

• Storage Conditions :

  CD20 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• CAS Number :

  9007-83-4

• Location :

  Predominantly cell surface with some cytoplasmic

• Image Legend :

  Flow cytometry testing of PFA-fixed human Raji cells with CD20 antibody (clone IGEL/773) ; Red=isotype control, Blue= CD20 antibody.