HMW Cytokeratin Antibody / Basic / Type II

• Description:

  This antibody recognizes HMW Keratin 1, 5, 10 and 14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. The HMW Cytokeratin antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this mAb. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. It has also been useful in separating benign from malignant intraductal breast proliferations.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Solubilized keratin extract from human stratum corneum was used as the immunogen for this HMW Cytokeratin antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  34BE12

• Applications:

  FACS, IF, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the HMW Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This HMW Cytokeratin antibody is available for research use only.

• Storage Conditions:

  Store the HMW Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  Western blot testing of human MCF7 cell lysate with HMW Cytokeratin antibody (clone 34BE12) .