Basic Cytokeratin Antibody

• Description:

  6.0) subfamilies. This antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1) ; 64kDa (CK3) ; 59kDa (CK4) ; 58kDa (CK5) ; 56kDa (CK6) ; 52kDa (CK8) . Many studies have shown the usefulness of keratin markers in cancer research and tumor diagnosis. The basic family clone AE3 is commonly used in combination with clone AE1 as a pan cytokeratin antibody acidic + basic cocktail (Cat No V2330) .Cat No V2330

• Specifications:

  Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Solubilized cytokeratin extract from human stratum corneum was used as the immunogen.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  AE3

• Applications:

  WB, FACS, IHC-P, IF

• Purity:

  Protein G purified

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the Basic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Basic Cytokeratin antibody AE3 is available for research use only. The formulation is not suitable for therapeutic or diagnostic testing.

• Storage Conditions:

  Store the Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  IHC: FFPE skin stained with Basic Cytokeratin antibody AE3.