CD45RB Antibody

• Description:

  CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out) . The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. antibody to CD45R is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-3 µg/mL, Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  The non-T, non-B CALLA positive ALL cell line REH (Leucocyte Workshop IV and V) was used as the immunogen for this antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  BRA-11

• Applications:

  FACS, IF, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  The CD45RB antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

• Limitations:

  This CD45RB antibody is available for research use only.

• Storage Conditions:

  The CD45RB antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Cell surface and cytoplasmic

• Image Legend:

  Flow cytometry staining of PFA-fixed human Raji cells with CD45RB antibody (clone BRA-11) ; Red=isotype control, Blue= CD45RB antibody.