CD45RA Antibody

• Description :

  CD45RA is an isoform of the human leukocyte common antigen (CD45) . Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve) .

• Specifications :

  Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

• UniProt :

  P08575

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Stimulated human leukocytes were used as immunogen for this CD45RA antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2a κ

• Clone :

  PTPRC/818

• Applications :

  FACS, IF, WB, IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1X PBS, pH 7.4

• Reconstitution :

  The CD45RA antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

• Limitations :

  This CD45RA antibody is available for research use only.

• Storage Conditions :

  The CD45RA antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number :

  9007-83-4

• Location :

  Cell surface

• Image Legend :

  IHC test of FFPE human tonsil probed with CD45RA antibody. HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 minutes.