IGF1 Antibody

• Description:

  This antibody is specific to IGF1 (Insulin like Growth Factor 1) and shows minimal cross-reaction with IGF11, Proinsulin, MSF, and Insulin. IGF1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF1B while isoform 2 is known as IGF1A. IGF1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of the IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.

• Specifications:

  Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, In-vitro neutralization of biological activity of IGF-1 (order BSA/sodium azide-free format)

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Purified human IGF1 protein was used as the immunogen for this antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  M23

• Applications:

  FACS, IF, Neutralization

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the IGF1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This IGF1 antibody is available for research use only.

• Storage Conditions:

  Store the IGF1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic (Secreted)

• Image Legend:

  SDS-PAGE Analysis of Purified, BSA-Free IGF1 Antibody (clone M23) . Confirmation of Integrity and Purity of the Antibody.