IGF1 Antibody

• Description :

  This antibody is specific to IGF1 (Insulin like Growth Factor 1) and shows minimal cross-reaction with IGF11, Proinsulin, MSF, and Insulin. IGF1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF1B while isoform 2 is known as IGF1A. IGF1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of the IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.

• CAS Number :

  9007-83-4

• Specifications :

  Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, In-vitro neutralization of biological activity of IGF-1 (order BSA/sodium azide-free format)

• Host :

  Mouse

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Purified human IGF1 protein was used as the immunogen for this antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  M23

• Applications :

  FACS, IF, Neutralization

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1X PBS, pH 7.4

• Reconstitution :

  Store the IGF1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This IGF1 antibody is available for research use only.

• Storage Conditions :

  Store the IGF1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

• Location :

  Cytoplasmic (Secreted)

• Image Legend :

  SDS-PAGE Analysis of Purified, BSA-Free IGF1 Antibody (clone M23) . Confirmation of Integrity and Purity of the Antibody.