IGF1R Antibody

• Description:

  IGF1R is a tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1) . Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1 receptor is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis.

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

• UniProt:

  P08069

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 51-77 from the human protein was used as the immunogen for this IGF1R antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC, FACS, IF, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the IGF1R antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This IGF1R antibody is available for research use only.

• Storage Conditions:

  Aliquot the IGF1R antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the IGF1R antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Mouse, Rat

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of IGF1R antibody and WiDr lysate. Predicted molecular weight: ~200 kDa (pro), 120-130 kDa (alpha), 90-97 kDa (beta) .