ErbB2 Antibody / HER2

• Description :

  This antibody recognizes a protein of 185kDa, which is identified as HER2 / ErbB2. Its epitope is localized in the extracellular domain. ErbB2 is a member of the EGFR family. This antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. ErbB2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

• Specifications :

  ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-3 µg/mL

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Recombinant human protein was used as the immunogen for this ErbB2 antibody. (1)

• Clonality :

  Monoclonal

• Isotype :

  IgG2a κ

• Clone :

  HRB2/258

• Applications :

  ELISA, FACS, IF

• Purity :

  Protein G purified

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Store the ErbB2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This ErbB2 antibody is available for research use only.

• Storage Conditions :

  Store the ErbB2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  Variations in protocols, secondaries and substrates may require the antibody to be titered for optimal performance.1. This ErbB2 antibody binds to the extracellular/cell surface region of the protein.

• CAS Number :

  9007-83-4

• Location :

  Extracellular/Intracellular cell membrane

• Image Legend :

  Immunofluorescent staining of methanol-fixed human SLBR3 cells with ErbB2 antibody (clone HRB2/258, green) and Reddot nuclear stain (red) .