HER2 Antibody / ErbB2

CAT: 0800-V2108-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V2108-100UGSize:100 µg
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb is specific to HER2 / ErbB2 and shows minimal cross-reaction with other members of the family. HER2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. HER2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
CAS Number
9007-83-4
Specifications
ELISA: order BSA/sodium azide-free format for coating, Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-4 µg/mL
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for this HER2 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
HRB2/451
Applications
ELISA, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the HER2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This HER2 antibody is available for research use only.
Storage Conditions
Store the HER2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the mAb to be titered up or down for optimal performance.
Location
Cell surface
Image Legend
Immunofluorescent staining of PFA-fixed human MCF-7 cells with HER2 antibody (green) and Reddot nuclear stain (red) .

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