CD74 Antibody

• Description:

  CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. CD74 antibody stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. antibody to CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+) .

• Specifications:

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Host:

  Mouse

• Reactivity:

  Human, Mouse

• Immunogen:

  SU-DHL-4 lymphoma cells were used as the immunogen for this CD74 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  LN-2

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the CD74 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD74 antibody is available for research use only.

• Storage Conditions:

  Store the CD74 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• CAS Number:

  9007-83-4

• Location:

  Cell surface and paranuclear globular

• Image Legend:

  IHC staining of FFPE human spleen tissue with CD74 antibody (clone LN-2) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.