CD74 Antibody

• Description:

  CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. CD74 antibody stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. antibody to CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+) .

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence (methanol fixed) : 1-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

• Host:

  Mouse

• Reactivity:

  Human, Mouse

• Immunogen:

  SU-DHL-4 lymphoma cells were used as the immunogen for this CD74 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  LN-2

• Applications:

  FACS, IF, IHC-P, WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the CD74 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD74 antibody is available for research use only.

• Storage Conditions:

  Store the CD74 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Cell surface and paranuclear globular

• Image Legend:

  Immunofluorescent staining of human Raji cells with CD74 antibody (clone LN-2, green) and Reddot nuclear stain (red) .