CD20 Antibody

• Description:

  This antibody recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12) . The antibody recognizes the extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of Bcells and its expression is restricted to normal and neoplastic Bcells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of Bcell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting Bcells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in Bcell activation and cell cycle progression.

• Specifications:

  Functional studies (order BSA/sodium azide-free format), Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  The lymphoblastoid cell line Daudi was used as the immunogen for this CD20 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  B9E9

• Applications:

  Functional studies, FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the CD20 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD20 antibody is available for research use only.

• Storage Conditions:

  Store the CD20 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD20 antibody to be titered up or down for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Predominantly cell surface with some cytoplasmic