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CD1b Antibody

CAT: 0800-V2028SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#: V2028SAF-100UGSize: 100 µg
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PRICE
AVAILABILITY
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes CD1b, a 44kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells. The CD1 multigene family encodes five forms of the CD1 T-cell surface glycoprotein in human, designated CD1A, 1B, 1C, 1D and 1E. CD1, a type 1 membrane protein, has structural similarity to the MHC class I antigen and has been shown to present lipid antigens for recognition by T lymphocytes. Constitutive endocytosis of CD1b molecules and the differential sorting of MHC class II from lysosomes separate peptide- and lipid antigen-presenting molecules during dendritic cell maturation. It is also expressed in interdigitating cells.
CAS Number
9007-83-4
Specifications
ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL
Host
Mouse
Reactivity
Human
Immunogen
Human peripheral lymphocytes were used as the immunogen for this CD1b antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
RIV12
Applications
ELISA, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the CD1b antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD1b antibody is available for research use only.
Storage Conditions
Store the CD1b antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD1b antibody to be titered up or down for optimal performance.
Location
Cell surface, cytoplasmic
Image Legend
FACS staining of human PBMCs using CD1b antibody (RIV12) .
Product Datasheet
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