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DDX6 Antibody

CAT: 0800-RQ6541Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6541Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
DDX6 (DEAD/H BOX 6), also known as HLR2 or p54, is an enzyme that in humans is encoded by the DDX6 gene. DDX6 belongs to the DEAD box family of putative RNA helicases that contain a characteristic asp-glu-ala-asp (DEAD) box motif (Seto et al., 1995) . Tunnacliffe et al. (1993) assigned the DDX6 gene more precisely using a panel of sequence tagged sites (STSs) representing 30 markers previously assigned to 11q23. Using mass spectroscopy, Fenger-Gron et al. (2005) found that RCK, EDC3 (YJDC), and HEDLS (RCD8) co-immunopurified with DCP1A and DCP2 from HEK293 cell lysates. Overexpression of DCP2, RCK, or EDC3 in HeLa cells reduced the association of endogenous DCP1A and XRN1 with cytoplasmic P bodies.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P26196
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
An E. coli-derived human protein (amino acids Q56-P483) was used as the immunogen for the DDX6 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
8G6
Applications
WB, IF, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the DDX6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This DDX6 antibody is available for research use only.
Storage Conditions
After reconstitution, the DDX6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the DDX6 antibody should be determined by the researcher.
Location
Cytoplasmic, nuclear
Image Legend
Flow cytometry testing of human HeLa cells with DDX6 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= DDX6 antibody.
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