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VASA Antibody / DDX4

CAT: 0800-R31067Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31067Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
DEAD/H BOX 4, also known as DDX4 and VASA contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al. (2000) mapped the gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse homolog resides (Abe and Noce, 1997) . Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al. (2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunohistochemistry (Frozen) : 0.5-1 µg/mL, Immunocytochemistry: 2 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL
UniProt
Q9NQI0
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 253-272 (EDEDSIFAHYQTGINFDKYD) were used as the immunogen for this VASA antibody (100% human, mouse and rat homology) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IHC-F, ICC, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the VASA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This VASA antibody is available for research use only.
Storage Conditions
After reconstitution, the VASA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the VASA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of VASA antibody and Lane 1: rat testis; 2: mouse testis; 3: HeLa cell lysate
Product Datasheet
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