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DDX1 Antibody

CAT: 0800-RQ6285Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6285Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ATP-dependent RNA helicase DDX1 is an enzyme that in humans is encoded by the DDX1 gene. It is mapped to 2p24.3. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL
UniProt
Q92499
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A human recombinant partial protein (amino acids K562-F740) was used as the immunogen for the DDX1 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
3I10
Applications
WB, IHC-P, FACS, IF
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the DDX1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This DDX1 antibody is available for research use only.
Storage Conditions
After reconstitution, the DDX1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the DDX1 antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic
Image Legend
IHC staining of FFPE mouse brain tissue with DDX1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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