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Trim25 Antibody / Efp

CAT: 0800-RQ6343Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6343Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Tripartite motif-containing protein 25 is a protein that in humans is encoded by the TRIM25 gene. It is mapped to 17q22. The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q61510
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
Recombinant mouse protein (amino acids M1-H590) was used as the immunogen for the Trim25 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the Trim25 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Trim25 antibody is available for research use only.
Storage Conditions
After reconstitution, the Trim25 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Trim25 antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic
Image Legend
IHC staining of FFPE mouse intestine tissue with Trim25 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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