TRIM29 Antibody

• Description:

  Tripartite motif-containing protein 29 interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA.

• Specifications:

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• UniProt:

  Q14134

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  An N-terminal human recombinant protein fragment was used as the immunogen for the TRIM29 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  TPMP29-1

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

• Reconstitution:

  Store the TRIM29 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This TRIM29 antibody is available for research use only.

• Storage Conditions:

  Store the TRIM29 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic & cell surface

• Image Legend:

  IHC testing of FFPE human esophageal carcinoma and TRIM29 antibody (clone TPMP29-1) . FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.