Cd20 Antibody

• Description :

  CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What�s more, this protein may be involved in the regulation of B-cell activation and proliferation.

• Specifications :

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

• UniProt :

  P11836

• Host :

  Rabbit

• Reactivity :

  Mouse

• Immunogen :

  Recombinant mouse protein (amino acids M1-P291) was used as the immunogen for the Cd20 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, IHC-P, FACS, Direct ELISA

• Purity :

  Antigen affinity purified

• Format :

  Purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution :

  After reconstitution, the Cd20 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This Cd20 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the Cd20 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the Cd20 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Cell surface, cytoplasmic

• Image Legend :

  IHC staining of FFPE mouse spleen tissue with Cd20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.