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IGF-1 Antibody

CAT: 0800-RQ6099Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6099Size:100 µg
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Description
Insulin-like growth factor 1 (IGF-1) also known as somatomedin C or mechano growth factor is a protein that in humans is encoded by the IGF1 gene. IGF-1 is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin is used for the treatment of growth failure. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7649 daltons.Justice et al. (1990) placed the mouse IGF1 gene on chromosome 10.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA (Capture) : 1-5 µg/mL
UniProt
P05019
Host
Rabbit
Reactivity
Human
Immunogen
A human recombinant partial protein (amino acids P50-A118) was used as the immunogen for the IGF-1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
IHC-P, FACS, ELISA (Capture)
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the IGF-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This IGF-1 antibody is available for research use only.
Storage Conditions
After reconstitution, the IGF-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the IGF-1 antibody should be determined by the researcher.
Image Legend
Flow cytometry testing of human HeLa cells with IGF-1 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= IGF-1 antibody.

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