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CD123 Antibody / IL3RA

CAT: 0800-RQ5983Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5983Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Interleukin 3 receptor, alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123), is a human gene. It is mapped to Xp22.33 and Yp11.2. The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5) . The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P26951
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human protein (amino acids T111-T378) was used as the immunogen for the CD123 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the CD123 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CD123 antibody is available for research use only.
Storage Conditions
After reconstitution, the CD123 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the CD123 antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
Flow cytometry testing of human Raji cells with CD123 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= CD123 antibody.

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