TRIM28 Antibody / KAP1

• Description:

  Tripartite motif-containing 28 (TRIM28), also known as transcriptional intermediary factor 1 beta (TIF1b) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE and Frozen) : 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt:

  Q13263

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant human protein (amino acids A699-P835) was used as the immunogen for the TRIM28 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a

• Clone:

  9E3.

• Applications:

  WB, IHC-P, IHC-F, IF, FACS

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the TRIM28 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This TRIM28 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the TRIM28 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the TRIM28 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  IHC staining of FFPE human breast cancer with TRIM28 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.