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TRIM28 Antibody / KAP1

CAT: 0800-RQ5933Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5933Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Tripartite motif-containing 28 (TRIM28), also known as transcriptional intermediary factor 1 beta (TIF1b) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE and Frozen) : 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
Q13263
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein (amino acids A699-P835) was used as the immunogen for the TRIM28 antibody.
Clonality
Monoclonal
Isotype
IgG2a
Clone
9E3.
Applications
WB, IHC-P, IHC-F, IF, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the TRIM28 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This TRIM28 antibody is available for research use only.
Storage Conditions
After reconstitution, the TRIM28 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the TRIM28 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
IHC staining of FFPE human breast cancer with TRIM28 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Product Datasheet
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