KAP1 Antibody / TRIM28

• Description:

  The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

• Specifications:

  Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

• UniProt:

  Q13263

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 178-207 from the human protein was used as the immunogen for the KAP1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IF, IHC-P, WB

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the KAP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This KAP1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the KAP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the KAP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  Western blot testing of human 1) HeLa and 2) PC-3 cell lysate with KAP1 antibody. Expectd molecular weight: 88~110 kDa depending on sumoylation level.