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ACADS Antibody / SCAD

CAT: 0800-RQ5790Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5790Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Acyl-CoA dehydrogenase, C-2 to C-3 short chain is an enzyme that in humans is encoded by the ACADS gene. This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P16219
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids EIQRLVIAGHLLRSYRS from the human protein were used as the immunogen for the ACADS antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the ACADS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ACADS antibody is available for research use only.
Storage Conditions
After reconstitution, the ACADS antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the ACADS antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human MCF7 cells with ACADS antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · P16219

Short-chain specific acyl-CoA dehydrogenase, mitochondrial

ACADS_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P16219
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ACADS
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
SCAD
EC number
1.3.8.1
Processing
Precursor
Secondary accessions
P78331
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Disease

Disease variant

Ligand

FADFlavoprotein

Biological process

Fatty acid metabolismLipid metabolism

Cellular component

Mitochondrion

Molecular function

Oxidoreductase

Domain

Transit peptide

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