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Cyclin B2 Antibody / CCNB2

CAT: 0800-RQ5675Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5675Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
G2/mitotic-specific cyclin-B2 is a protein that in humans is encoded by the CCNB2 gene. This gene is mapped to 15q22.2. Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
O95067
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein (amino acids K23-A391) was used as the immunogen for the Cyclin B2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Cyclin B2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Cyclin B2 antibody is available for research use only.
Storage Conditions
After reconstitution, the Cyclin B2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Cyclin B2 antibody should be determined by the researcher.
Image Legend
IHC staining of FFPE human ovarian cancer with Cyclin B2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

UniProtKB · O95067

G2/mitotic-specific cyclin-B2

CCNB2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O95067
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CCNB2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B3KM93, Q6FI99
Protein keywords

Biological process

Cell cycleCell divisionMitosis

Molecular function

Cyclin

PTM

Phosphoprotein

Technical term

Proteomics identificationReference proteome

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