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SDHA Antibody

CAT: 0800-RQ5669Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5669Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Succinate dehydrogenase complex, subunit A, flavoprotein variant is a protein that in humans is encoded by the SDHA gene. It is mapped to 5p15.33. This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P31040
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids YRPVIDKTLNEADCAT from the human protein were used as the immunogen for the SDHA antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the SDHA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This SDHA antibody is available for research use only.
Storage Conditions
After reconstitution, the SDHA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the SDHA antibody should be determined by the researcher.
Image Legend
Western blot testing of human 1) HeLa, 2) HepG2, 3) HEK293, 4) Caco-2 and rat 5) brain, 6) kidney, 7) spleen, 8) liver and mouse 9) brain, 10) kidney, 11) liver lysate with SDHA antibody. Predicted molecular weight ~72 kDa.
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