SDHC Antibody

• Description:

  The Succinate dehydrogenase complex, subunit C (SDHC), also called SDH3, gene contains 6 exons. Oostveen et al. (1995) found that in fact it was protein from the bovine SDHC/SDH3 gene (encoding 1 of the 2 integral membrane proteins) that complemented the hamster mutation. The authors localized the human SDH3 gene to the short arm of chromosome one, within 1 to 2 Mb from the centromere. Additionally, the authors stated that Southern analyses of human genomic DNA suggested that there are multiple SDH3 genes or pseudogenes. Elbehti-Green et al. (1998) confirmed the assignment of the SDHC gene to 1q21 by FISH. Baysal et al. (2004) described a family with PGL3 in which an 8,372-bp deletion in the SDHC gene was transmitted both maternally and paternally, without evidence of genomic imprinting.

• Specifications:

  Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

• UniProt:

  Q99643

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  An amino acid sequence from the N-terminus of human Succinate dehydrogenase complex subunit C (KEEMERFWNKNIGSNR) was used as the immunogen for this SDHC antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution:

  After reconstitution, the SDHC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This SDHC antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the SDHC antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the SDHC antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of SDHC antibody and Lane 1: rat liver; 2: HeLa; 3: Jurkat cell lysate