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NCAM1 Antibody

CAT: 0800-RQ5592Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5592Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NCAM is a membrane-bound glycoprotein that plays a role in cell-cell and cell-matrix adhesion through both its homophilic and heterophilic binding activity. The neural cell adhesion molecule appears on early embryonic cells and is important in the formation of cell collectives and their boundaries at sites of morphogenesis. Later in development it is found on various differentiated tissues and is a major CAM mediating adhesion among neurons and between neurons and muscle. NCAM gene is mapped to 11q23. The neural cell adhesion molecule (NCAM) can influence a number of diverse intercellular events, including junctional communication, the association of axons with pathways and targets, and signals that alter levels of neurotransmitter enzymes.
CAS Number
9007-83-4
Specifications
Western blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P13591
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A human recombinant protein (amino acids K152-Q690) was used as the immunogen for the NCAM1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the NCAM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This NCAM1 antibody is available for research use only.
Storage Conditions
After reconstitution, the NCAM1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the NCAM1 antibody should be determined by the researcher.
Image Legend
Western blot testing of 1) rat brain, 2) rat C6 and 3) mouse brain lysate with NCAM1 antibody. Predicted molecular weight: ~110 kDa (soluble fragment), ~120/125 kDa (GPI-anchored), 140/180 kDa (transmembrane isoforms) .

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