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NCAM Antibody

CAT: 0800-V4202-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V4202-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas) . It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma) . Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P13591, P13592
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human NCAM1 protein was used as the immunogen for the NCAM antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RNCAM1/8655
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the NCAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This NCAM antibody is available for research use only.
Storage Conditions
Aliquot the NCAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the NCAM antibody should be determined by the researcher.
Location
Cell surface
Image Legend
IHC staining of FFPE human colon tissue with CD56 antibody (clone rNCAM1/8655) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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